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The Biuret Test
| Test | Observation | Inference |
| Sample + NaOH(aq) solution + CuSO4(aq) solution in drop, followed by thorough shaking + warm | Violet or purple precipitate formed | Sample is protein |
The Millons test
| Test | Observation | Inference |
| Sample solution + millions reagent+ heat | White precipitate is formed. Precipitate turns deep red or brick red on heating. | Protein is present |
The Xanthoproteic Test
| Test | Observation | Inference |
| Sample + conc. HNO3(aq) in drops + heat + few drops of NaOH(aq) or NH4OH(aq) | Yellow precipitate, which turns orange on addition of NaOH(aq) or NH4OH(aq) | Sample is protein |
Responses